目的 对生脉注射液所含化学成分进行全面、系统的定性研究。方法 分别采用柱前衍生-气质联用、高分离度快速液相-离子阱-多级质谱、离子交换色谱-柱后衍生化等技术对样品中糖、皂苷、木脂素、氨基酸等成分进行了鉴别,并采用分子排阻色谱-蒸发光散射法对大分子物质的检查做出了初探。结果 生脉注射液中含有果糖、葡萄糖、蔗糖、麦芽糖、5-羟甲基糠醛、19种皂苷、14种木脂素和17种氨基酸。结论 所建方法简便、准确,基本阐明了该品种的化学物质基础,为后续单个成分的含量测定研究提供了实验基础。
Abstract
OBJECTIVE To conduct a comprehensive and systematic qualitative investigation on chemical components in Shengmai injection. METHODS Gas chromatography-mass spectroscopy (GC-MS) with pre-column derivatization, rapid resolution liquid chromatography-ion trap-tandem mass spectroscopy (RRLC-IT-MSn) and ion exchange chromatography (IEC) with post-column derivatization were applied in identification of saccharides, saponins, lignins and amino acids in the sample. Furthermore, preliminary investigation on test of macromolecules was performed using size exclusion chromatography-evaporative light scattering detection (SEC-ELSD). RESULTS Fructose, glucose, sucrose, maltose, 5-hydroxymethyl-2-furaldehyde, 19 saponins, 14 lignins and 16 amino acids were found in Shengmai injection. CONCLUSION The proposed methods are simple and accurate. The main chemical substantial basis of Shengmai injection is clarified, laying a foundation for further assay of chemical components.
关键词
生脉注射液 /
物质基础 /
定性分析 /
皂苷 /
木脂素 /
糖 /
氨基酸
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Key words
Shengmai injection /
substantial basis /
qualitative analysis /
saponin /
lignin /
saccharide /
amino acid
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中图分类号：
R284
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参考文献
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脚注
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基金
国家自然科学基金项目资助(81303194);中国食品药品检定研究院学科带头人培养基金项目资助(2017X1)
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